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KMID : 0624620120450010051
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BMB Reports
2012 Volume.45 No. 1 p.51 ~ p.56
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Glucocorticoid treatment independently affects expansion and transdifferentiation of porcine neonatal pancreas cell clusters
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Kim Ji-Won
Sun Cheng-Lin
Jeon Sung-Yoon
You Young-Hye
Shin Ju-Young
Lee Seung-Hwan
Cho Jae-Hyoung
Park Chung-Gyu
Yoon Kun-Ho
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Abstract
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The purpose of this study was to determine the effects of duration and timing of glucocorticoid treatment on the expansion and differentiation of porcine neonatal pancreas cell clusters (NPCCs) into β-cells. After transplantation of NPCCs, the ductal cyst area and β-cell mass in the grafts both showed positive and negative correlations with duration of dexamethasone (Dx) treatment. Pdx-1 and HNF-3β gene expression was significantly downregulated following Dx treatment, whereas PGC-1α expression increased. Pancreatic duct cell apoptosis significantly increased following Dx treatment, whereas proliferation did not change. Altogether, transdifferentiation of porcine NPCCs into β-cells was influenced by the duration of Dx treatment, which might have been due to the suppression of key pancreatic transcription factors. PGC-1α plays an important role in the expansion and transdifferentiation of porcine NPCCs, and the initial 2 weeks following transplantation of porcine NPCCs is a critical period in determining the final β-cell mass in grafts.
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KEYWORD
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Dexamethasone (Dx), Diabetes mellitus, Neonatal porcine pancreas cell clusters (NPCCs), Pancreatic β-cells, Transdifferentiation
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